2290 Exam Question

2290 Exam Question - Sample Exam Questions: Zabulionis...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Sample Exam Questions: Zabulionis Unit: FALL/10: It is in your best interest to try the questions first before looking at the answers; this gives you a chance to see how your thinking works for these types of questions. 1. Ampicillin plates are prepared to a final "working concentration" of 100 μg/mL. If the stock solution of amp is 10 mg/mL, how much antibiotic stock solution would be mixed with how much medium to achieve the working concentration in 1 litre final volume? (Show your work.) (3 marks) 2. If an overnight culture was diluted to 10 -7 and then 278 colonies were counted after plating 100µL, what is the estimated number of cells per mL in this overnight culture? (Show your work for part marks.) (4 marks) 3. In the Zabulionis Unit, we stated that the Prediction is a statement of what one thought would happen in an experiment. The Conclusion was a short summary of the Data and how it related to the Prediction. Given the Data and Conclusion, in one or two sentences, what is the Prediction of this experiment? Data: Growth or number of transformants after transformation of E. coli JM101 with increasing amounts of TE buffer or pGREEN plasmid. Amount of TE Buffer Plate Type Growth 10 μL LB confluent 10 μL LB + amp no 20 μL LB confluent 20 μL LB + amp no 30 μL LB confluent 30 μL LB + amp no Amount of pGREEN plasmid Plate Type Number of Transformants 10 μL LB + amp 123 20 μL LB + amp 238 30 μL LB + amp 364 Conclusion: In the transformation of E. coli JM101 with increasing amounts of pGREEN plasmid (10 μL to 30 μL) there was an increase in the number of transformants which agrees with the prediction. (3 marks)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4. Some mutants of
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/01/2011 for the course BIOLOGY Biology 22 taught by Professor Dr.dean,krajnyk,zabulionis during the Fall '10 term at UWO.

Page1 / 4

2290 Exam Question - Sample Exam Questions: Zabulionis...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online