Chapter 3 Learning Objectives

Chapter 3 Learning Objectives - C hapter 3 Learning...

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Chapter 3 Learning Objectives 1. 2. Amino acids: a. They are compounds containing an amino group, -NH 2 , and a carboxylic acid group, -COOH. b. Ionic properties: c. Titration curves i. pK 1 ii. pK 2 iii. pK R iv. pI (pH I ) d. salt/ acid ratio e. Henderson- Hasselbalch equation 3. Be able to distinguish D- and L-forms with Fischer a. All the naturally occurring amino acids have the right-hand structure in this diagram. This is known as the "L-" configuration. The other one is known as the "D-" configuration. 4. Recognize the UV absorption characteristics of three amino acids Absorption Fluorescence Amino Acid Wavelength (nm) Absorbtivity Wavelength (nm) Quantum Yield Tyrophan 280 5600 348 0
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.20 Tyrosine 274 1400 303 0.14 Phenylalani ne 257 200 282 0.04 5. Peptide bonds are formed between the carboxyl group of one amino acid and the amino group of the next amino acid. Peptide bond formation occurs in a condensation reaction involving loss of a molecule of water. 6.
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Chapter 3 Learning Objectives - C hapter 3 Learning...

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