Methods Section Assignment

Methods Section Assignment - and E staining. They were then...

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Tanisha Billups BIO 120 November 15, 2010 Methods Section Assignment The convention in writing a methods section is to use paragraph structure not “recipe” structure. DO NOT LIST materials. DO NOT give numbered order of steps. Write the paragraph in past tense using the following writing style. In addition to the description of what was done in the experiment you need to write a separate paragraph (SHORT ONE) on what two stains were used: SPECIAL NOTE: PLEASE PROVIDE THE ACTUAL NAMES OF STAINS #1 and #2. They were not mentioned in the protocol but they are commonly known as H and E staining. Cells, which have been grown attached to cover slips, were stained using H
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Unformatted text preview: and E staining. They were then mounted using a mounting medium to a microscope slide. Once the slides were dry, the cells were observed at 100X and 400X magnification using a compound microscope. H and E stain or hematoxylin and eosin stain, is a widely used method of staining in histology. Actually, it is the most popular stain. The H and E stain is chosen for its ability to stain a variety of cellular components of tissue. The hematoxylin stains nuclei of cells blue while eosin is an acidic dye with an affinity for cytoplasmic components of the cell and stains in shades of red, pink, and orange....
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