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Genetic Reprogramming

Genetic Reprogramming - BME100:Topic10 Yamanaka,Nature2009...

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4/26/2011 1 BME 100: Topic 10 Genetic Reprogramming Yamanaka, Nature 2009 modified from Conrad Waddington, 1957
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4/26/2011 2 S Huang, Bioessays 2009 S Huang, Bioessays 2009
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4/26/2011 3 S Huang, Bioessays 2009 Genetic Reprogramming Pluripotent Stem Cell Fibroblast (skin) Myoblast (muscle) Osteoblast (bone)
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4/26/2011 4 Genetic Reprogramming Pluripotent Stem Cell MyoD Runx2 Fibroblast (skin) Myoblast (muscle) Osteoblast (bone) Genetic Reprogramming Pluripotent Stem Cell c-myc Klf4 Sox2 Oct4 MyoD Runx2 Fibroblast (skin) Myoblast (muscle) Osteoblast (bone) MyoD Runx2
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4/26/2011 5 Genetic Reprogramming • Altering a cell’s state or lineage by changes in gene expression Hi t • History: • Somatic Cell Nuclear Transfer Remove the nucleus from an oocyte and replace it with the nucleus from a differentiation cell • 1952 – Frogs • 1997 - Dolly the sheep Cell Fusion - 1983 • Direct reprogramming between lineages with transcription factors • 1989 – MyoD • 2006 – Induced pluripotent stem cells (iPS cells) Genetic Reprogramming Gurdon & Melton, Science 2008
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4/26/2011 6 Genetic Reprogramming Gurdon & Melton, Science 2008 Gurdon & Melton, Science 2008
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