DISCUSSION2 - 4 DISCUSSION In the experiment of Aqueous Two...

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4. DISCUSSION In the experiment of Aqueous Two Phase System, as it is also explained in the introduction part, the aim is to separate PEG 4000 and potassium phosphate. For this purpose, in the laboratory, firstly we put 1 mL, 2 mL, 3 mL, 4 mL, 5 mL and 6 mL PEG 4000 40% (w/w) stock solution in 10 mL test tubes. Until observing one phase, we add potassium phosphate in small amounts by mixing perfectly. The samples of 3 mL, 4 mL, 5 mL and 6 mL have successful results, whereas one phase does not observed in 1 mL and 2 mL samples although 8 mL potassium phosphate is added. Afterwards, the steps are repeated for potassium phosphate. We put 2 mL, 3 mL and 4 mL solution of potassium phosphate 20% (w/w) in 10 mL test tubes. Only addition of 3.5 mL PEG 4000 to 2 mL sample gives the expected result of one phase. For obtaining more accurate binodal curve, we dilute the PEG 4000 stock solution to 50% (v/v), 33.3% (v/v), 66.6% (v/v) and 40% (v/v). Their mass fractions (w/w) are found and showed in appendix. One phase is observed by adding small
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This note was uploaded on 05/01/2011 for the course CHE che 320 taught by Professor Ahmetarslan during the Spring '11 term at Middle East Technical University.

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DISCUSSION2 - 4 DISCUSSION In the experiment of Aqueous Two...

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