lecture 7

lecture 7 - RNA synthesis Gene Transcription 701...

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701 RNA synthesis Gene Transcription
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702 Gene mRNA Translation at ribosome Protein Transcription Transcription: DNA -> RNA
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703 mRNA: messenger RNAs tRNA: transfer RNAs rRNA: ribosomal RNAs snRNA: small nuclear RNAs miRNA: micro RNAs gRNAs: guide RNAs all RNAs derive from genes! all RNAs are synthesized by transcription!
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704 (RNA) n+1 + PP i 2P i (RNA) n + NTP H 2 O The Reaction Initiation Elongation Termination DNA + RNA Three phases
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705 E.coli RNA polymerase: Holoenzyme initiates: α 2 ββ σ Core Enzyme elongates: α 2 ββ Zn 2+ Mg 2+
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706
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707 “hand” shape with channel that fits B-DNA thumb may close channel T7 RNA polymerase: <100kd E. coli RNA polymerase: >400kd
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708 RNA Polymerase from Taq ß-subunit ß’-subunit α -subunit α -subunit Mg 2+ Zn 2+
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709 MgCl 2+
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Phosphodiester bond formation in RNA polymerase active site 3’-OH attacks α -phosphoryl group of incoming nucleoside triphosphate
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… but subunit organization and sequence has diverged
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lecture 7 - RNA synthesis Gene Transcription 701...

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