BIO151 Exam2_Review_Spring11

BIO151 Exam2_Review_Spring11 - filtration, MPN, and direct...

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BIO151 Microbiology Exam 2 on Chapter 6 and 7 Topics Covered You should understand all topics discussed in class. Use class notes, outlines, and textbook images to prepare for exam. Here is a list of the topics covered: Ch 6- Microbial Growth -Physical Requirements (Temp, pH, and Osmotic Pressure) -Chemical Requirements (C, N, S, P, Trace Elements, Oxygen, and Organic) -Effect of Oxygen on Growth -Biofilms -Terms: Culture medium (pl. media), Sterile, Inoculum, Culture, Agar, Chemically defined media, complex media -Anaerobic Culture Methods (Thioglycolate, Anaerobic Jar/Chamber) -Capnophile -Biosafety Levels -Selective media, differential media, and enrichment culture -How to obtain a pure culture using the streak plate method -Terms: Colony, Colony-forming unit, streak plate method -Preserving bacterial cultures -Binary fission -Generation Time -Bacterial Growth curve and (4) phases of growth (lag, log, stationary, and death) -Direct Measurements of bacterial growth (dilution and plating, membrane
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Unformatted text preview: filtration, MPN, and direct microscopic count) -Indirect Measurements of bacterial growth (turbidity, metabolic activity, and dry weight) Ch 7-Control of Microbial Growth-Terms: sepsis, asepsis, sterilization, disinfection, antisepsis, degerming, sanitization, biocide/germicide, bacteriostasis -Microbial Death Curve -Factors that determine how effective treatment is -Mode of action for microbial control agents -Physical Methods of Microbial Control: TDP vs, TDT, moist heat sterilization, autoclave (steam sterilization), pasteurization, dry heat, filtration, low temperature, high pressure, desiccation, osmotic pressure, and radiation -Chemical Methods of Microbial Control: phenol/phenolics, bisphenols, biguanides, halogens (iodine, tincture vs. iodophor), alcohols, heavy metals, surfactants, food preservatives, aldehydes, gaseous sterilants, and peroxygens. -Ranking of microbes from most resistant to least resistant -Use-Dilution Test -Disk-Diffusion Test...
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This note was uploaded on 05/03/2011 for the course BIO 151 taught by Professor Mansour,a during the Spring '11 term at Bunker Hill.

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