Lab4_Gel - fast. The linear plasmid moves at about the same...

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23,130 bp Relaxed circular plasmid 9,416 bp 6,557 bp Different stages of coiling 4,361 bp linear (cut) plasmid 2,322 bp 2,027 bp supercoiled plasmid 564 bp Remember, movement is based on both SIZE and SHAPE, as well as net charge, which isn't a major factor here. The RNAs are numerous, small, and of varying sizes. Thats why they produced the large 'smear'. The supercoiled plasmid is small, so it moves
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Unformatted text preview: fast. The linear plasmid moves at about the same rate as the DNA ladder (it is also linear). Plasmids in various stages of coiling move slower than linear, and the relaxed circular plasmid moves slowest. Based on this, can you tell which column is from a blue colony, a white colony, or both? RNA...
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This note was uploaded on 05/05/2011 for the course BIOL 400 taught by Professor Dr.biology during the Spring '11 term at University of Tennessee.

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