restriction mapping - 5/5/11 Blunt vs sticky ends 5/5/11...

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5/5/11 Gel Electrophoresis Size and charge
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5/5/11
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5/5/11
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Click to edit Master subtitle style 5/5/11 Restriction Mapping
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5/5/11 Restriction enzymes Also called restriction endonucleases Normally found and isolated from bacteria. WHY?
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5/5/11 Nomenclature Named from organism in which they were discovered:
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5/5/11 Two types Type I: cut at random sites Type II: recognize a very specific sequence and cut within recognition site
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5/5/11 EcoRI: recognizes a
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Unformatted text preview: 5/5/11 Blunt vs sticky ends 5/5/11 5/5/11 Use of restriction enzymes? 5/5/11 DNA ladder 5/5/11 Restriction mapping 5/5/11 Restriction mapping: tips recognize that DNA can be circular or linear Tally the number of restriction sites for each of the enzymes by examining the number of fragments produced . With linear DNA the number of fragments is always 1 more than the number of sites; with circular DNA, the...
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This note was uploaded on 05/05/2011 for the course BIOL 400 taught by Professor Dr.biology during the Spring '11 term at University of Tennessee.

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restriction mapping - 5/5/11 Blunt vs sticky ends 5/5/11...

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