2011lecture1pwrpt - Lecture Week 1 Biology 242 Spring 2010 11 Dr Susan Karcher G-225 Lilly Hall Email [email protected] 22 Lab manual 2011

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5/6/11 11 Lecture Week 1 Biology 242 Spring 2010
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5/6/11 Dr. Susan Karcher G-225 Lilly Hall Email [email protected] 22
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5/6/11 Lab manual 2011 University Bookstore 1. Molecular Genetics 2. Bacterial Genetics 3. Classical Genetics 33
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5/6/11 Some exercises/techniques to master—titre Some multi-week experiments, build upon prior week work 44
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5/6/11 BIOLOGY 242 Purdue University 2011 schedule Dr. Sue Karcher, G-225 Lilly Hall, X48083 Debbie Anderson, 1-112 Lilly Hall, X63896 55
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5/6/11 Schedule in lab manual Expectations in lab manual 66
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5/6/11 Biology 242 Spring 2011 Grading Policy A = 87.00-100% B = 74.00-86.99% C = 60.00-73.99% D = 45.00-59.99% F = Below 45.00% Total 350 points 77
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5/6/11 Note: The above grading cutoffs will be used. There will not be any “lowering of the curve.” Notify TAs in writing of any questions about grades within 1 week from the posting of the grades online. 88
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5/6/11 Exams and Quizzes These will be given in the Monday lecture for all sections at once. Quiz 1 25 pts Week 4 Quiz 2 25 pts Week 7 Quiz 3 25 pts Week 11 Quiz 4 25 pts Week 13 Exam 1 50 pts Week 9 Exam 2 50 pts. Week 15 99
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5/6/11 In lab, during week 15, there will be a make-up quiz given for missing a quiz with an excused absence. 1010
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5/6/11 Lab work Notebook checks in the lab section Check 1 5 pts Week 4 Check 2 10 pts Week 8 Check 3 10 pts Week 15 TA points TAs award these points based on: being on time to lab; being prepared; response to oral questions 25 pts 1111
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5/6/11 Written Papers Note: If material does not have a formal write up required, it will be covered in notebook and quizzes. Cloning paper 20 pts Week 7 Southern paper 10 pts Week 9 Bioethics talk 10 pts Week 5, 6, & 7 Bioethics paper 10 pts Week 8 Fly progress report 20 pts Week 12 Fly final paper and exercises 30 pts Week 14 1212
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5/6/11 How to succeed in Biology 242: Read Prepare ahead Time/plan Resources Notebook: spiral bound okay Bring own: Sharpie;Ruler;Scissors;Tape 1313
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5/6/11 Open lab Partner Plan experiment 1414
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5/6/11 Absences Document Excuse 3 unexcused absences --fail course Plagarism—zero, fail 1515
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5/6/11 Week 1 Read lab 1 in manual Find partner; Meet TAs Find lab drawer Review sterile technique; review streak for single colonies Why? Preparation for cloning lab 1616
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5/6/11 Week 2 Begin cloning lab Select bioethics topics week 1, revise week2 1717
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5/6/11 1818 Hints-- will mention in lecture today : 1. Be able to name 3 of the 6 features of an E.coli host cell to use with pUC. 2. Be able to name the features of a cloning vector. 3. Be able to sketch the pUC vector. Not worried about drawing it to scale.
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5/6/11 1919 Features of a cloning vector 1. Unique RE sites pUC has msc— multiple cloning site 2. Unique RE within an “indicator gene”-- something that can be screened, selected or scored. pUC has lacZ’ gene for blue/white screening Earlier types of cloning vectors had antibiotic resistance genes.
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5/6/11 2020 3.
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This note was uploaded on 05/06/2011 for the course BIOL 242 taught by Professor Staff during the Spring '08 term at Purdue University-West Lafayette.

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2011lecture1pwrpt - Lecture Week 1 Biology 242 Spring 2010 11 Dr Susan Karcher G-225 Lilly Hall Email [email protected] 22 Lab manual 2011

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