Problems chapter 5

Problems chapter 5 - 18 Which of the following compounds...

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Chem 365 Homework problems Chapter 5 1-15. From Voet, Voet & Pratt (VVP), Chapter 5: Problems 1-10 and 14-18 (pages 121-123). 16. In SDS-PAGE a mixture of proteins is combined with the ionic detergent sodium dodecyl sulfate. This normalizes the shape of the proteins and gives them all a net _____________ charge. Subsequently, the protein mixture is loaded onto a polyacrylamide gel and an electric field is applied. This separates the individual proteins based only on their ________. a. positive, isoelectric point b. negative, size c. amphipathic, color d. neutral, amino acid sequence 17. Treatment of the peptide sequence DTLFSMKDEMYEKQQWTERGMSALHL with the protease trypsin will yield what peptides?
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Unformatted text preview: 18. Which of the following compounds reduces (breaks) disulfide bonds in proteins? a. !-mercaptoethanol b. Cyanogen bromide c. Trypsin d. Urea 19. The enzyme gyrase is a tetramer composed of two subunits of molecular weight 110,000 and two subunits of molecular weight 90,000. A sample of the pure gyrase is run on a gel filtration column under physiological conditions (i.e., neutral pH, normal saline). A single peak corresponding to molecular weight 400,000 is detected. A separate sample of gyrase is electrophoresed on a polyacrylamide gel containing SDS. What is the expected outcome of the SDS PAGE? Explain your answer....
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