Unformatted text preview: 18. Which of the following compounds reduces (breaks) disulfide bonds in proteins? a. !-mercaptoethanol b. Cyanogen bromide c. Trypsin d. Urea 19. The enzyme gyrase is a tetramer composed of two subunits of molecular weight 110,000 and two subunits of molecular weight 90,000. A sample of the pure gyrase is run on a gel filtration column under physiological conditions (i.e., neutral pH, normal saline). A single peak corresponding to molecular weight 400,000 is detected. A separate sample of gyrase is electrophoresed on a polyacrylamide gel containing SDS. What is the expected outcome of the SDS PAGE? Explain your answer....
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- Spring '08
- molecular biology, SDS-PAGE, a. b. c., b. c. d., Polyacrylamide gel