365 S2011 L14 - Characterizing O2-binding ! Mb + O2 MbO2...

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1 Characterizing O 2 -binding Mb + O 2 MbO 2 k = [Mb][O 2 ] [MbO 2 ] Y O 2 = [MbO 2 ] [MbO 2 ]+[Mb] Y O 2 = [O 2 ] k + [O 2 ] = pO 2 k + pO 2
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2 K is equal to ligand concentration 50% saturation
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3 Y O 2 = pO 2 k + pO 2 When k = pO2 Y O 2 = k k + k = 0.5
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4 Myoglobin Small protein, carries heme (solubility) Heme is the actual O 2 carrier Fe 2+ is octahedrally coordinated Myoglobin prevents irreversible oxydation of Fe (II) It helps coordinate Fe (II) Provides a binding space for O 2 , and may prevent other molecules from binding Binding is characterized by k
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5 Octahedron
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6
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7 Why do we need blood to transport O 2 ? Why do we need hemoglobin? Why does hemoglobin have 4 subunits?
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8 In organisms larger than 2 mm diffusion is to slow to supply the tissues with O 2 We need hemoglobin to increase O 2 solubility of blood
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9 Hemoglobin
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10 Hemoglobin transports O 2 from the lungs to the tissues Take up as much O 2 in the lungs as possible Leave as much O 2 behind as possible in the tissues AfFnity?
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11 Sigmoid binding curve
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O 2 -binding curves for hemoglobin and myoglobin
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365 S2011 L14 - Characterizing O2-binding ! Mb + O2 MbO2...

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