365 S2011 L21 - Movement of molecules across a membrane...

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1 Movement of molecules across a membrane resembles a chemical reaction
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2 Δ G A = G A (in) G A (out) Δ G A = G ° A + RT ln A [ ] in - G ° A + RT ln A [ ] out ( ) Δ G A = RT ln A [ ] in A [ ] out R = gas constant, 8.314 J/°K.mol
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3 Equilibrium is affected by the ionic charge and differences in electrical potential
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4 R = gas constant 8.314 J/°K.mol Z = ionic charge of A F = 96485 C/mol ΔΨ = Ψ in - Ψ out membrane potential V 1V = 1 J/C Δ G A = RT ln A [ ] in A [ ] out + Z A F ΔΨ Change in free energy for transport of 1 mol A from the outside to the inside
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5
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6 Transport Non-mediated transport non-polar molecules Mediated transport polar and charged molecules
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7 Mediated transport 1. Passive or facilitated diffusion From high to low concentration 2. Active From low to high concentration Requires energy (ATP)
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8 Passive transport 1. Carriers 2. Porins 3. Channels 4. Transport proteins
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9 Ionophores
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10 • Cyclic molecule • Contain 12 building blocks
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365 S2011 L21 - Movement of molecules across a membrane...

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