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Unformatted text preview: a. -mercaptoethanol b. Cyanogen bromide c. Trypsin d. Urea 19. The enzyme gyrase is a tetramer composed of two subunits of molecular weight 110,000 and two subunits of molecular weight 90,000. A sample of the pure gyrase is run on a gel filtration column under physiological conditions (i.e., neutral pH, normal saline). A single peak corresponding to molecular weight 400,000 is detected. A separate sample of gyrase is electrophoresed on a polyacrylamide gel containing SDS. What is the expected outcome of the SDS PAGE? Explain your answer. You will see two bands. One at 110 kDa, representing the large subunit, and one at 90 kDa representing the small subunit. You will see both bands because boiling in SDS denatures proteins and interferes with the interactions that stabilize non-covalent bonds between the subunits....
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- Spring '08