BIO325 Exam 2 - ‘@ng{3de<9 gigging prmfl 20H 1 In the...

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Unformatted text preview: __ ‘ @ng {3de <9 gigging prmfl 20H 1. In the dihybrid cross shown below, genes A and B behave according to Mendel's two laws. In addition, allele A is completely dominant to a, allele B is completely dominant to b, and the A and B genes affect different traits. P AABB x aabb Fl AaBb (self) Ah ab F2 A. What are the gamete genotyges produced by the F1? Circle your answer (5 pts.) B. At what frequency is each gamete type in question A above produced? Circle our answer (5 pts.) C. How would your answer to question 3 above change if the alleles do not obey Mendel's 2"d law because they are linked with a recombination frequency of 10%? Circle your answer (15 pts.) RF: [0% meant» [f (Ab) +f(a5)]= /(7% /0% of 1&7ka are rflcmbmfifil firm] 2. Consider the Following testcross (A and B control differe t traits. A is completely dominant to a, and B is completely dominant to )2 Ab/aB X ab/ab l 1; Ab / ab 4999 aB / ab 4999 AB/ b l REab/sb 1 A. What is the recombination Frequency (RF) between the A and B genes? Circle your answer. (5 pts.) 0 cl 0 9- ‘ 03’ == 0 094 /o ; —_'_’_———- f,- /o,ooo [000 /00 8. During a typical meiosis (in other words, in most meiosis events). how many cross-overs occur between the A and 8 genes? (5 pts.) (Your answer should be an inte er - circle it.) -JEQ / a fin Man of Mel‘asfi @ SW5 2221" wi aPsrijngle, X—over @6111ch A+ B C. Are the A and B genes on the same chromosome?. No (circle one) (5 pts.) 4 [ ex/M) D. IF you self-cross Ab/aB, what would be he frequency/\of each of the F2 phenolypic classes corresponding to the geno’rypic classes listed below? Circle your answers (10 p’rs. ) A_ bb r? 3 boxes m (WW); +2< 000M “1) _....—....._. ”WM/M a ,Mw ”rm mmmwflmlflm ”whom—am! m C} 3 19%.? xiii?“ 1“ 10% aabb *1 box A (.0000; 3. Four homozygous mutant lines oF Fruit Flies (labeled 1-4) each have the same mutant phenotype: Four wings instead oF the normal two. Each line is homozygous For an amorphic mutation in a single gene. The lines were intercrossed, and the phenotypes oF the resulting F1 Flies are shown in the Following grid. “+” indicates that the F1 had two wings, and “-" indicates that the F1 had Four wings. males 1 2 3 4 u JWS mm... are in Me 1 _ + + + éaMEfjel’lt— A: 2 + — _ ’ + 3Com Iementa 0 f0 S E’ 3 + — — + g| 4 + + + — o 1.3 .4 A. What is the one word describes this kind oF test? Circle your answer. (5 pts) C Coil“ Ekmelfl éfiofi D B. How many diFFerent genes are mutant among the 4 Ii 5? (10 pts) (your answer should be an integer - circle ”0(3); C. Which lines contain mutations in the some genes? Circle our answers. (10 pts) C —y————— and 3 D. From the description oF the alleles and the mutant phenotype above, what would you inFer is the normal Function oF these genes? E. IF the alleles that caused the mutant phenotype described above were neomorphic, what would you inFer about the normal Function oF the genes? 794/16th of a "TWP“ 4. You are growing a strain of auxotrophic Ieu- (cannot synthesize Leucine) E. coli bacteria in a flask of minimal media supplemented with Leucine. A. How can you identify Ieu+ prototrophs that may have arisen by spont us mu ? words allowed onl - circle them) (5 pts.) grow colonies on mi‘mmaL me [a B. Is the method you pro ose in A a screen or a selection? (5 pts.) Circle one: screen (selection) C. Suppose that you started with a strain of wild-type (prototrophic) E. cali growing in a flask containing complete media (minimal media supplemented with all 20 amino acids). You add some EMS (a mutagen) to the culture. Then you transfer 200 bacteria from the flask to a Petri plate containing complete medium. In order to identify Ieu- auxotrophs that may be among the 200 resulting colonies, you need to perform a replica plating experiment using two different kinds of Petri plates. What are two kinds of plates that you need? Circle your answer. (10 pts) —— minima zrmimmai ,0"!S Leucuuo D. Is the method in C a screen or a selection? (5 pts.) Circle one: screen selection BONUS QUESTION Consider the pedigree diagram below. The affected individuals in the diagram have a disease carried by a dominant allele of anautosomal gene, D. The disease phenotype is 100% penetrant. The disease gene is linked to the gene I that controls ABO blood type. £53363; A. Circle the sym ols for the people in the diagram who are most likely recombinants. 8. Using all of the data in this diagram, calculate the recombination Frequency between the I and D genes. Circle our answer. RF; '/|3 (Wm are /3 data Points in this PEG/gift? (filaamm’ i3 progeny: In whom n" (’Zg‘fwggn possible +0 ObSQH/é’ rem/n - *H’le I find D genes.) ...
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