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NTR366L_spring2011_week3_HW_03_Day1

NTR366L_spring2011_week3_HW_03_Day1 - Lane 7 Empty Draw the...

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NTR 366L Spring 2011 Name, eid: Date: Unique No. Homework 3.1 1. (7 pts) Sketch a schematic of a gel that shows the results after PCR for some unspecified gene, followed by digestion with a restriction endonuclease: Lane 1: Empty Lane 2: 200-bp DNA ladder, bands from 200 bp to 1200 bp (omit any higher bands) Lane 3: undigested PCR product (student #1), 900 bp Lane 4: digested PCR product (student #1), 250 bp, 650 bp, 900 bp Lane 5: undigested PCR product (student #2), 900 bp Lane 6: digested PCR product (student #2), 250 bp, 650 bp
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Unformatted text preview: Lane 7: Empty Draw the outline of the gel, show the location of the wells, label each and every band with its size in basepairs (bp), and indicate the placement of the positive and negative electrodes. The spacing of the bands should demonstrate the relationship between fragment length and migration distance. 2. (1 pt) What do you think are the genotypes for students #1 and #2? 3. (2 pts) Sketch a graph showing the accumulation of PCR product with cycle number and label the 3 phases....
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