Unformatted text preview: synthesizes a double stranded DNA copy of the genomic DNA template. 3. (2 pts) In addition to reverse transcriptase and RNA template, what other components must be present for synthesis of cDNA? You need different primers 4. (2 pts) The reaction mixture contains an RNase inhibitor. List at least 2 things that you should do to protect the RNA in the sample from degradation by RNases. Two different ways to protect the RNA in the sample are wearing new gloves and using sterile tubes and pipette tips....
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This note was uploaded on 05/10/2011 for the course NTR 366L taught by Professor Zhu during the Spring '11 term at University of Texas.
- Spring '11