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NTR366L_spring2011_week8_HW02

NTR366L_spring2011_week8_HW02 - During the firs step of PCR...

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NTR 366L Spring 2011 Name, eid: Thu Hoang, tah 798 Date: Unique No. Homework 8.2 1. (2 pts) Why are there so many immunological variants of HIV? HIV has a higher error rate, therefore the epitopes frequent mutations change viral proteins 2. (2 pts) What is missing in the control reaction? What do you predict the results will be? Missing control reaction is PCR pellet and the predicted results are since there is no PCR rxn pellet, nothing will occur until it is added. 3. (2 pts) What do you think happens to the reverse transcriptase during the first step of PCR?
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Unformatted text preview: During the firs step of PCR, reverse transcriptase is activated. RNA is reverse transcribed to cDNA using reverse transcriptase. 4. (4 pts) Explain why the RNA sample will appear as a smear on the agarose gel. Explain why the cDNA (if run on the gel before the PCR was carried out) would look the same. RNA will be smeared because it is partially degraded RNA and will lack rRNA bands cDNA looks the same because...
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