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Unformatted text preview: During the firs step of PCR, reverse transcriptase is activated. RNA is reverse transcribed to cDNA using reverse transcriptase. 4. (4 pts) Explain why the RNA sample will appear as a smear on the agarose gel. Explain why the cDNA (if run on the gel before the PCR was carried out) would look the same. RNA will be smeared because it is partially degraded RNA and will lack rRNA bands cDNA looks the same because...
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This note was uploaded on 05/10/2011 for the course NTR 366L taught by Professor Zhu during the Spring '11 term at University of Texas at Austin.
- Spring '11