NTR366L_spring2011_week7_lecture

NTR366L_spring2011_week7_lecture - Todays Topics:...

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1 Today’s Topics: Immunodiffusion techniques Transgenic animals
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2 Immunodiffusion technique Uses a specific antibody to detect the presence of an antigen Antibody and antigen bind to multiple sites on each other When amounts of antigen and antibody are equivalent, an insoluble precipitate forms Uses diffusion of antibody and/or antigen through agar Qualitative and quantitative assays
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3
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4 Equivalence
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5 Ouchterlony Analysis Double diffusion technique Antibody and antigen are placed in wells in agar Diffuse toward each other At some point in between the wells the concentrations reach equivalence If the antibody reacts with the antigen, a thin white line of precipitin appears Takes ~24 hrs Qualitative test
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6 Mancini Analysis Radial diffusion test Antibody is incorporated in the agar Known amounts of antigen and the test sample are placed in wells in the agar Each sample diffuses outward from the well in a circle The greater amount of antigen, the farther it has to
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NTR366L_spring2011_week7_lecture - Todays Topics:...

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