NTR366L_spring2011_TakeHome2AlphabetSoup

NTR366L_spring2011_TakeHome2AlphabetSoup - NTR 366L...

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NTR 366L Take-home Lab #2 Due Week of Feb 28, Lab Day 2 Thu Hoang NTR 366L:545454 Alphabet Soup: PCR, SNPs, and RFLPs in Nutrition Research Part 1 1. What are the sections of a typical scientific paper? Summary or abstract, introduction, materials and methods, results, discussion, acknowledgements, references, figures and tables 2. List three types of research and the questions that they ask. Descriptive–What is there? What do we see? Comparative–How does it compare to other organisms? Are our findings general? Analytical-How does it work? What is the mechanism? 3. Define the abbreviations PCR, SNP, and RFLP. PCR is polymerase chain reaction SNP is single nucleotide polymorphism RFLP is restriction fragment length polymorphism 4. Define and compare and contrast “mutation” and “polymorphism.” A mutation is induced from outside the cell by an exogenous factor, inheritable, and may not be harmful to the host. Polymorphism is induced within the cell, changes through evolution, heritable, and may also not be harmful to the host. 5. List the steps to follow to detect SNPs by PCR-RFLP analysis. (Hint: This is how you determined your PTC tasting genotype.) Students amplified a specific sequence of DNA by PCR. It is then digested with a restriction enzyme called HaeIII. Students determine their genotype by running their products on a 2% agarose gel, which established their ability to taste PTC. 6. Distinguish between “nutrigenetics” and “nutrigenomics”. Nutrigenetics is the relationship between genotype and the risk of developing diet related diseases like type II diabetes Nutrigenomics is the study of the effects of food on gene expression. Part 2 1. State the hypothesis tested. (Hint: See the Abstract.) The hypothesis is genes involved in lipid metabolism, i.e. the genes implicated in intestinal uptake, intracellular trafficking and the lipoprotein distribution of lipids, play a role in the plasma concentrations of these micronutrients Page 1 of 4
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NTR 366L Take-home Lab #2 Due Week of Feb 28, Lab Day 2 2. What are carotenoids and what are their purported roles in human health? Carotenoids are commonly found in human diets. They are fat soluble micronutrients
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NTR366L_spring2011_TakeHome2AlphabetSoup - NTR 366L...

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