NTR366L_spring2011_Takehome4microarray - NTR 366L Take-home...

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NTR 366L Take-home Lab #4 Due Lab Day 2, Week of April 11, 2011 Microarrays and Personal Genomics: the Next Frontier Goals: 1.) To learn more about designing an experiment using gene expression microarrays. 2.) To learn more about personal genomics. Due date: Please turn in the answers to Parts IV and VI at the beginning of the lab on which it is due (April 14, 15, or 16). Please note that your grade will be reduced by 20% for every day that it is late. Also note that these are discussion questions; there are few right or wrong answers, but you must be able to support your arguments. Just for fun, see http://www.biocompare.com/video/illumina/genetic_analysis/ This animation pits two different types of microarray “platforms” (Affymetrix and Illumina) against each other. Gene Expression Analysis using Microarrays Northern blots and RT-PCR are two techniques commonly used to analyze gene expression, i.e., the levels of mRNA transcribed from particular genes in a given cell or tissue. Usually, the goal of the experiment is to determine how a treatment or condition affects mRNA levels (up or down). Typically, with northern blots expression of only one gene at a time is determined. In contrast, with microarrays the expression of thousands of genes or all genes (the “transcriptome”) can be assessed simultaneously. Part I . Watch these simple introductions to reverse transcriptase-PCR (RT-PCR) and DNA microarrays from Davidson College Genome Consortium for Active Teaching: http://www.bio.davidson.edu/courses/genomics/RTPCR/RT_PCR.html http://www.bio.davidson.edu/courses/genomics/chip/chip.html (Be aware that this is a demonstration of how cDNA microarrays are processed and that procedures using microarrays made of oligonucleotides differ in certain details.) Part II
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This note was uploaded on 05/10/2011 for the course NTR 366L taught by Professor Zhu during the Spring '11 term at University of Texas.

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NTR366L_spring2011_Takehome4microarray - NTR 366L Take-home...

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