NTR366L_spring2011_week4_HW1 - 4. (2 pts) What will happen...

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NTR 366L Spring 2011 Name, eid: Date: Unique No. Homework 4.1 1. (1 pt) Why must gloves be worn while handling the membrane for a Southern blot? 2. (2 pts) Why does the probe bind to a specific DNA sequence? 3. (3 pts) List the conditions that must be correct for hybridization to occur appropriately.
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Unformatted text preview: 4. (2 pts) What will happen if there are bubbles between the gel and the membrane during the transfer? 5. (2 pts) For a circular piece of double-stranded DNA containing 4 recognition sites for a restriction endonuclease, how many DNA fragments will be present after digestion?...
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This note was uploaded on 05/10/2011 for the course NTR 366L taught by Professor Zhu during the Spring '11 term at University of Texas.

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