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NTR366L_spring2011_week7_Immunodiffusionprotocol

NTR366L_spring2011_week7_Immunodiffusionprotocol - Lab Day...

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Lab Day 1 and 2, Week of March 28, 2010 Immunodiffusion Techniques: Meat Adulteration Tests Goal: To illustrate immunodiffusion techniques by testing for adulteration of beef with horsemeat. Brief description of the experiment: This laboratory will demonstrate two immunodiffusion techniques, a qualitative test (a double diffusion technique called the Ouchterlony Analysis) and a quantitative test (a single radial diffusion test called the Mancini Analysis). Both are based on the principle that a white precipitate will form when a specific antibody binds its antigen in the right proportions. Both tests use diffusion of reagents in agar to detect the presence of beef or horse albumin, an abundant serum protein. In the Ouchterlony Analysis the test sample is placed in a well in the agar and allowed to diffuse toward other wells containing anti-bovine albumin antibody and anti-horse albumin antibody; a white precipitin line should appear between the test sample and the anti-bovine albumin antibody, but a white precipitin line will appear between the test
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