microbiology flash cards

microbiology flash cards - Serial dilution Tube Dilution...

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Serial dilution Tube Dilution Original Culture: 1ml #1: 99 ml H2O (after shaking 20x) using new pipette 99 ml H20 1ml into #2: 99 ml H2O (after shaking the H2O 20x) using new pipette
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#2: 99 ml H20 1 ml #1: 9 ml test tube using new pipette #1: 9 ml tube 1ml #2: 9 ml test tube #2: 9 ml tube 1 ml #3: 9 ml test tube #3: 9 ml tube #4: 9 ml test tube #1: 9 ml tube .01 ml into #1: 10(-6) agar plate using new pipette #2: 9 ml tube 1.0 ml in #2: 10(-6) agar plate; and 0.1 ml into #3: 10(-7) agar plate #3: 9 ml tube 1.0 ml into #4: 10(-7) agar plate; and 0.1 ml into #5: 10(-8) agar plate
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#4: 9 ml tube 1.0 ml into #6: 10(-8) agar plate Dilution factor: Tube dilution + additional dilution if plating 0.1 ml (i.e. 10(-5) tube dilution + plate 0.1 ml = 10(-6) final dilution on plate) Incubate 24 hours at 30 e C CFU/ml. = # of colonies counted (tube dilution)(volume plated) What does
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TFTC stand for ? Too Few To Count (too few colonies to count)
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What does TNTC Stand for? Too Numerous To Count
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(too many colonies to count) What is considered too few colonies?
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Fewer than 30 colonies is considered TFTC.
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What is considered too many colonies to count? More than 300 colonies is considered too many to count.
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What are the steps for making a streak plate for Isolating Pure Cultures? 1. Choose a mix of two bacteria (i.e. E.coli & Marcescens)
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2. Suspend organisms in broth by flicking the upright tube 3. Take a loopful of culture from your Mix and deposit the drop of culture in surface Area 1, flame loop. 4. Quench (cool) loop in Area 4, drag it several times across the area of surface 1. Flame loop. 5. Rotate plate 90e degrees. 6. Quench loop in Area 4, touch loop to corner of Area 1 and drag it several times across agar surface in Area 2. Flame loop. 7. Rotate plate 90e degrees.
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Area 2, drag it several times across the agar surface in Area 3. 9. Rotate plate 90e . 10. WithOUT flaming the loop drag culture from corner in Area 3 across Area 4 using a wider streak. Flame loop.
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This note was uploaded on 05/11/2011 for the course BIO 31 taught by Professor Silva during the Spring '11 term at Reedley.

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microbiology flash cards - Serial dilution Tube Dilution...

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