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Proximate Analysis_Blank

Proximate Analysis_Blank - lipids Calculation based on...

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1 Proximate Analysis Proximate Analysis ± Combination of Analytical Procedures: Germany, 1860 { Moisture (dry matter) { Ash { Crude Protein { Crude Fat (ether extract) { Crude Fiber { Nitrogen-free Extract ***No relationship to nutritional value, other than diagnostic*** Proximate Analysis ± Moisture/DM { Heat sample above boiling point of water ± End wt./Initial wt.*100 = DM or ± 100 - %H 2 O = %DM Proximate Analysis ± Ash (mineral component) { heat sample for 6h at 600 o C Proximate Analysis ± Crude Protein (CP) { Acid digestion destroys organic matter and releases Nitrogen (N) as ammonium sulfate. { Neutralized with NaOH, distilled to release free ammonia, which is captured with a standard acid (boric acid). { Titrated with dilute base and quantity measured to calculate %N.
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2 Proximate Analysis Crude Protein, % = %N x 6.25 Amino acids, on average, contain 16% N 100/16 = 6.25 Proximate Analysis ± Ether Extract (EE) { Sample extracted with ether to remove
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Unformatted text preview: lipids { Calculation based on weight difference Crude Fiber ± Crude Fiber { After EE fractions are removed: 1. Weak Acid 2. Weak Base 3. Ashed – cellulose, lignin burned, ash remains Proteins/sugars/starches removed Crude Fiber (Extracted and acid/bassic digested sample – ash residue) Initial sample weight x 100% = %CF 3 Nitrogen-Free Extract (NFE) ± NFE (nonstructural sugars/starches) ± No chemistry, just arithmetic 100 – (%H 2 O + %CP + %EE + %CF + %Ash) Gross Energy. .. ± Determined by bomb calorimetry Energy I. Definition A. The energy content of feedstuffs 1. In animal nutrition, we are interested in the chemical (molecular) energy of feedstuffs 2. Heat energy can be determined by bomb calorimetry 3. One calorie raises the temperature of 1 g of water 1°C (i.e., 14.5 to 15.5 °C ); a kilo-calorie (kcal) raises 1000 g or water 1 °C...
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Proximate Analysis_Blank - lipids Calculation based on...

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