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Unformatted text preview: 15. Explain how the blue/white screening works. What is X-gal? Betagalactosidase? What color are the mutant and wild types and why? 16. What is sequencing? What is the name of the common sequencing method? 17. Why would you want to synthesize DNA? 18. Explain PCR. Why is it done? 19. What are Taq polymerase and Pfu polymerase? 20. What is RT-PCR and qPCR? 21. What is site-directed mutagenesis? 22. What are the common hosts for cloning vectors? 23. How do antibodies and nucleic acid probes help identify clones? 24. Why is the phage M13 a good cloning vector? 25. What kinds of genes are cloned?...
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