Ch._12_-_Study_Questions - 15. Explain how the blue/white...

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Biol 261 – Ch. 12 – Study Questions 1.  Describe the basic techniques used in genetic engineering. 2. What is a restriction enzyme?  Give two examples. 3. How do restriction enzymes know where to cut the DNA? 4. How is a sticky end different from a blunt end? 5. Why don’t restriction enzymes cut the bacterial DNA? 6. Why do scientist use gel electrophoresis?  Ethidium bromide? 7. What is a restriction map?  Selection marker? 8. What is a nucleic acid probe?  9. What is a Southern and Northern blot? 10. What is molecular cloning?  How is it done?  Why is it done? 11. What is a vector?  Give examples.   12. What is DNA ligase? 13. How does one get DNA into a cell? 14. What is pUC19?  Why is it so useful?
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Unformatted text preview: 15. Explain how the blue/white screening works. What is X-gal? Betagalactosidase? What color are the mutant and wild types and why? 16. What is sequencing? What is the name of the common sequencing method? 17. Why would you want to synthesize DNA? 18. Explain PCR. Why is it done? 19. What are Taq polymerase and Pfu polymerase? 20. What is RT-PCR and qPCR? 21. What is site-directed mutagenesis? 22. What are the common hosts for cloning vectors? 23. How do antibodies and nucleic acid probes help identify clones? 24. Why is the phage M13 a good cloning vector? 25. What kinds of genes are cloned?...
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Ch._12_-_Study_Questions - 15. Explain how the blue/white...

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