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Practice Final with Discussion Answers

Practice Final with Discussion Answers - R L Rodriguez...

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R. L. Rodriguez, Instructor BIS101-001, Spring 2008, Final Exam June 12, 2008 Please print your name below and each subsequent page. Your exam has 11 questions and 9 pages (including cover page and score sheet). AUTHORIZATION FOR POSTING OF GRADES BY SOCIAL SECURITY OR STUDENT ID NUMBER I, ______________________________, student ID No. ____________________ authorize the University to post my grades in BIS101-001 identified by my student I.D. or Social Security number. Signed: ________________________________ Dated: _______________ ------------------------------------------------------------------------------------------------------------------- AUTHORIZATION FOR PUBLIC DISTRIBUTION OF GRADED PAPERS OR EXAMINATIONS I, _______________________________, student ID No. ____________________ authorize the University to distribute publicly my graded papers, homework assignments, and examinations (e.g., handed out in class or left in a exam bin) for BIS101-001 Signed: ________________________________ Dated: _______________
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Name____________________________________________________ 2 1. The figure below shows a single nucleotide polymorphism ( SNP ) between two alleles that creates a BamHI restriction fragment length polymorphism (RFLP) in a 10 kb stretch of chromosomal DNA. The RFLP can be detected by Southern blot analysis using a radiolabeled probe complementary to this region. Because this SNP is associated with the Sickle Cell Anemia, individuals can be screened for this autosomal recessive disease. Three individuals submitted their DNA for genetic testing to determine their risks for the disease. The Southern blot analysis reveals the following bands. Individual 1 1.5kb Individual 2 1.5kb and 4kb Individual 3 4kb (a) Which individual will definitely get the disease? (5 pts.) (b) Which individual is a carrier of the disease allele? (5 pts.) (c ) Which individual is not a carrier of the disease allele? (5 pts.) (d) If a probe corresponding to the 8kb region were used instead of the one corresponding to the 6.5 kb region, what new banding pattern would be indicative of a carrier of the disease. Circle the correct answer(s) below. (5 pts.) 1.5kb 2.5kb 4 kb
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Name____________________________________________________ 3 2. The Wobble Hypothesis states that the third base at the 5' side of the anticodon loop can hydrogen bond with certain noncomplementary bases. The Wobble rules are shown below. Using these rules, determine the minimum number of anticodons (i.e. tRNAs) that can recognize all six arginine codons.
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