BIS101-003PracMidterm2

BIS101-003PracMidterm2 - Practice Midterm 2 BIS 101-003...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Practice Midterm 2 BIS 101-003 R.L. Rodriguez Please print your name below and each subsequent page. Your exam has 9 questions and 8 pages (including cover page and score sheet). 1. In prokaryotes, several enzymes are responsible for replicating chromosomal DNA. The table below shows enzymatic activities for these enzymes. Using plus (+) and minus (-), indicate which enzymes are associated with which activities. (7 pts.) Activities Pol I Primase Pol III Ligase 5’-3’ exonuclease 3’-5’ exonuclease 5’-3’ deoxynucleotide synthesis ATP-dependent 3’-5’ joining 5’-3’ ribonucleotide synthesis E. coli DNA polymerase makes approximately 9 errors per million bases synthesized while Taq DNA polymerase makes 285 errors per million bases synthesis. Which of the above enzymatic activities is responsible for this difference? (3 pts.) 2. As can be seen in the steps below, the polymerase chain reaction (PCR) involves the cycling of DNA and primers between different temperatures (in degrees Celsius). In the blanks below, fill in the temperature best suited for a particular step. (8 pts.) Step 1: Melting. The mixture is heated at _________for 5 minutes to ensure that the DNA strands as well as the primers have melted. The DNA-Polymerase can be present at initialization, or it can be added after this step. Step 2: Annealing. By reducing temperature to _______ for 30 seconds primers can anneal to target sequence in single stranded DNA. Step 3: Elongation. If DNA polymerase is present, deoxynucleotides can be elongation at ________ for 45 seconds. Step 4 : Termination. Mixture is held at _______ until it can be analyzed by gel electrophoresis.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Name____________________________________________________ 2 3 . In the PCR reaction, temperature is used to denature the double strands of target sequence
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 6

BIS101-003PracMidterm2 - Practice Midterm 2 BIS 101-003...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online