{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

BIS101-003PracMidterm2

BIS101-003PracMidterm2 - Practice Midterm 2 BIS 101-003 R.L...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Practice Midterm 2 BIS 101-003 R.L. Rodriguez Please print your name below and each subsequent page. Your exam has 9 questions and 8 pages (including cover page and score sheet). 1. In prokaryotes, several enzymes are responsible for replicating chromosomal DNA. The table below shows enzymatic activities for these enzymes. Using plus (+) and minus (-), indicate which enzymes are associated with which activities. (7 pts.) Activities Pol I Primase Pol III Ligase 5’-3’ exonuclease 3’-5’ exonuclease 5’-3’ deoxynucleotide synthesis ATP-dependent 3’-5’ joining 5’-3’ ribonucleotide synthesis E. coli DNA polymerase makes approximately 9 errors per million bases synthesized while Taq DNA polymerase makes 285 errors per million bases synthesis. Which of the above enzymatic activities is responsible for this difference? (3 pts.) 2. As can be seen in the steps below, the polymerase chain reaction (PCR) involves the cycling of DNA and primers between different temperatures (in degrees Celsius). In the blanks below, fill in the temperature best suited for a particular step. (8 pts.) Step 1: Melting. The mixture is heated at _________for 5 minutes to ensure that the DNA strands as well as the primers have melted. The DNA-Polymerase can be present at initialization, or it can be added after this step. Step 2: Annealing. By reducing temperature to _______ for 30 seconds primers can anneal to target sequence in single stranded DNA. Step 3: Elongation. If DNA polymerase is present, deoxynucleotides can be elongation at ________ for 45 seconds.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern