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Unformatted text preview: I HAVE USED THIS ASSIGNMENT! IF YOU USE IT YOU WILL BE CAUGHT! Homework Assignment-10 Due Monday November 15, 2010 This homework assignment is based on part of the assigned reading for Mondays class (p. 303-310). Since we are one class period behind where we should be, we will be covering more material in Mondays class than originally planned. So by completing this assignment before class, we should be able to spend less time in class discussing restriction enzymes and restriction mapping and be able begin discussing DNA cloning. Question I. What are restriction enzymes and how were they discovered? What do the terms restriction site and restriction fragment mean? A restriction enzyme is one that recognizes a specific sequence of bases in a DNA molecule anywhere within the genome and then severs two covalent bonds (one of each strand) in the sugar phosphate backbone at particular positions within or near said sequence. By studying bacterial cells and the viruses that infect them, scientists discovered that during restriction (when a bacterial cell is preventing a virus from replicating inside of it) viral DNA is broken into pieces and degraded. The enzyme was identified as an endonuclease. endonuclease....
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