01&02 - How do we approach studying gene expression and

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How do we approach studying gene expression and function/performance of certain proteins? 1. Biochemical approach – start from the protein (which is isolated by/ through its function or activity) – go towards DNA 2. Genetic approach – start from the gene - DNA (normal vs. mutated) – go towards production of protein (normal vs. mutated) Links between (or tools for both) genetic and biochemical approaches: recombinant DNA technology bioinformatics
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Biochemical approaches: protein to gene Genetic approaches: gene to protein
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Enables us to isolate/separate protein of interest based on its function/activity (example: RNAPs; keep it in mind as we will talk about it later in the course) The protein activity could be assayed under different conditions The ultimate goal is to deduce the sequence of the gene which codes for extracted protein
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Identification of mutants genetic screens essential genes and proteins absolutely necessary for survival Conditional lethal mutations permissive/nonpermissive conditions (e.g. temperature) information?
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Example : Isolating Temperature Sensitive Mutants in Yeast
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Genetic approach enables us to get the answers to following questions: 1. What is a potential function and “importance” of the protein? through identification / isolation / survival of MUTANTS 2. What is # of genes involved in certain process? 3. What is the order of these genes (proteins) in the process? by applying genetic approach called COMPLEMENTATION ANALYSIS 4. What are the interactions between different genes (proteins)? by using genetic approach called GENETIC SUPPRESSION
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Recombinant DNA technology : Combination of techniques Restriction enzymes: “cutting and pasting” DNA pieces Sequencing of DNA Nucleic acid hybridization DNA cloning DNA engineering Isolate, sequence and manipulate individual genes from any type of cell Enabled studies of structure and function of genes and the role of proteins in cell processes
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Bioinformatics What is Bioinformatics? Computer science applied in answering biology questions
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This note was uploaded on 05/16/2011 for the course BIOL 308 taught by Professor Miskovic during the Spring '09 term at Waterloo.

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01&02 - How do we approach studying gene expression and

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