05 - Reassociation is inversely proportional to the genome...

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Reassociation is inversely proportional to the genome (DNA) size Reassociation is inversely proportional to the genome (DNA) size . . If a genome does not contain repetitive sequences (for example, MS-2, T4 and E. coli genomes), the complexity of the genome (expressed in # of nucleotides) is (theoretically) the same as the genome size (Lewin, Genes VIII) The DNA sources are: synthetic DNA duplex of poly A and poly U polynucleotide chains; MS-2 DNA (bacteriophage); T4 DNA (more complex bacteriophage then MS-2); E . coli DNA; ( adapted from Britten, R. J., and Kohne, D. E., 1968. Science 161: 529– 540.)
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Determining Genome Size by Cot Analysis Determining Genome Size by Cot Analysis Calculate the ratio between the rate of renaturation (Cot 1/2 ) of known unique sequence DNA and that of the unique fraction of unknown test sample. From the Graph: The whole known is unique, Cot 1/2 is 5 60% of test genome is unique Cot 1/2 for this unique part is 1000 9
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CoT analysis CoT analysis recently revived: recently revived: aids genome aids genome sequencing sequencing Unique, protein coding sequences could be “purified” from the mix faster sequencing cheaper sequencing
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Original Britten and Kohne, 1968 Kinetics: Britten and Davidson, 1976
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N = haploid chromosome # C = DNA/haploid cell C varies greatly General increase from prokaryotes to eukaryotes Large differences within eukaryotes More DNA present than we can account for. .. C Value Paradox
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This note was uploaded on 05/16/2011 for the course BIOL 308 taught by Professor Miskovic during the Spring '09 term at Waterloo.

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05 - Reassociation is inversely proportional to the genome...

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