DNA.1 - DNA The Genetic Material Griffith and Bacterial...

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1 DNA: The Genetic Material 1 Griffith and Bacterial Transformation Streptococcus pneumoniae polysaccharide Streptococcus pneumoniae polysaccharide capsule = virulence factor S-strain (encapsulated)-lethal to mice • R-strain-nonpathogenic 2 The Griffith Mixed Culture Experiment 3 Fig. 2.2 Nature of the Transforming Material: Avery, MacLeod, and McCarty 1. Heat kill S-strain 2. Detergent lyse bacteria and separate cytosol from cellular debris 3. Treat cell extract with enzymes to degrade 4 proteins, lipids and carbohydrates 4. Mix enzyme treated cytosol with R-strain and examine colony morphology
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