Replication

Replication - DNA Replication It has not escaped our notice...

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1 DNA Replication “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the enetic material” 1 g -Watson and Crick DNA Replication Models 2 Fig 3.1 Meselson and Stahl Experiment •G row E. coli for many generations in 15 N media 4 • Transfer to 14 N for successive generations • Following each round of replication, separate DNA with cesium chloride gradient • Determine whether DNA molecules are heavy, ght or hybrid 3 light or hybrid 4
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2 5 Griffiths Fig 8-13 (See Russell F. 3.1 Replication in E. coli is Bidirectional Rodriguez 6 Griffiths Fig. 8-18 (See Russell Fig 3.9) Replication Forks DNA Synthesis Runs 5’ to 3’ OH 3’ 5’ P 5’ P OH 3’ 7 Major Replication Genes /Proteins DNA initiator complex -denatures strands, opens replication bubble dnaB /DNA Helicase – extends replication forks gyrA B /DNA Gyrase -relieves supercoiling ahead of replication fork. Gyrase is a type II topoisomerase
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This note was uploaded on 05/17/2011 for the course MCDB 101A taught by Professor Thrower during the Spring '08 term at UCSB.

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Replication - DNA Replication It has not escaped our notice...

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