Transc_Transl

Transc_Transl - 1 Transcription and Translation 1 mRNA...

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Unformatted text preview: 1 Transcription and Translation 1 mRNA Colinear with gene sequence Codes for one or more proteins One DNA strand serves as coding strand for a given mRNA (has same sequence as mRNA) Other strand is template strand (has a sequence omplementary that of the corresponding 2 complementary to that of the corresponding mRNA) mRNA is synthesized 5'-3' Both DNA Strands Can Serve as a Template for mRNA Trun Fig 12.2 3 Griffiths Fig 10-6 Genes Consist of Regulatory and Coding Sequences 4 Fig 5.3 2 RNA Polymerase Core enzyme: , ' 2 Holoenzyme: Core + sigma factor ( ) Sigma factor binds to promoter and localizes core enzyme to transcription initiation site 5 Trun Fig 12.3a Promoters are DNA Sequences Where the RNA Polymerase Holoenzyme Binds to Initiate Transcription Pribnow box 6 Trun Fig 12-3 E. coli Promoter Consensus Sequences 7 Griffiths Fig 10.8 Correct Spacing Between The Pribnow and -35 box is Required for Polymerase 8 y Binding Fig 3.3 Molecular Genetics of Bacteria by Dale & Park 3 Transcription A . Initiation 1. RNA polymerase binds to promoter ( closed configuration ) 2. Polymerase unwinds DNA helix ( open configuration ) 3. Nucleotides are incorporated (...
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Transc_Transl - 1 Transcription and Translation 1 mRNA...

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