MCDB101A Midterm 2 Study Guide Definitions: polycystronic mRNA, operon, constitutive vs. induced gene expression, positive vs. negative gene regulation, prophage/lysogen, virulent vs. temperate phage, phage immunity, antiterminator, mutation rate vs. mutation frequency, multiplicity of infection (MOI), point mutation, deletion, micro vs. macrolesions, transition vs. transversion mutation; types of mutations: neutral, silent, missense, and null/nonsense, intragenic vs. extragenic mutations, mutagen vs. carcinogen, regulon, homologous recombination, heteroduplex DNA, Chi sequence (know function, don’t memorize sequence) Concepts:How are X-gal and IPTG used to detect β-galactosidase production? What is a gel shift assay and how was it used to show that LacI binds to lacO? Know the order of dominance among Lac operon mutations Understand how CAP regulates lactose metabolism and how this fits in with regulation based on LacI How does the blue-white assay work and how is it used in cloning a gene?
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