MCDB101A Midterm 2 Study Guide
: polycystronic mRNA, operon, constitutive vs. induced gene expression, positive vs. negative
gene regulation, prophage/lysogen, virulent vs. temperate phage, phage immunity, antiterminator,
mutation rate vs. mutation frequency, multiplicity of infection (MOI), point mutation, deletion, micro vs.
macrolesions, transition vs. transversion mutation; types of mutations: neutral, silent, missense, and
null/nonsense, intragenic vs. extragenic mutations, mutagen vs. carcinogen, regulon, homologous
recombination, heteroduplex DNA, Chi sequence (know function, don’t memorize sequence)
How are X-gal and IPTG used to detect
What is a gel shift assay and how was it used to show that LacI binds to
Know the order of dominance among Lac operon mutations
Understand how CAP regulates lactose metabolism and how this fits in with regulation based on LacI
How does the blue-white assay work and how is it used in cloning a gene?
What factors influence whether phage lambda will undergo lysogeny?