Recombination

Recombination - Learning Objectives What are the functions...

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1 The Molecular Basis of Recombination 1 Learning Objectives • What are the functions of recombination? • What is the evidence that a physical transfer of DNA occurs during recombination? • What are the basic steps of the Holliday model of homologous recombination? • Why do only half of all recombination events generate a recombinant phenotype? • How do mismatches help to validate the Holliday dl? 2 model? • How does the Meselson-Radding model of recombination differ from the Holliday model? • What are the functions of the proteins that participate in homologous recombination? Functions of Recombination ypass of damaged bases during replication Bypass of damaged bases during replication • Rescue of collapsed replication forks that result when a DNA polymerase encounters a single-strand nick • Repair of DNA double-strand breaks 3 •Ex change of genetic information between different DNA molecules that share regions of homology The Meselson-Weigle Experiment • Differentially labeled two phage λ strains with heavy and light isotopes of carbon and nitrogen • Coinfected E. coli with both labeled strains • Collected phage from bacterial lysate and centrifuged through CsCl 2 • Multiple bands of phage were produced.
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Recombination - Learning Objectives What are the functions...

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