Review_Session2_Part1

Review_Session2_Part1 - MCDB 101A: Midterm 2 Review Session...

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MCDB 101A: Midterm 2 Review Session Presented by: Abhi Iyer and Suzanne Gelber- 11/13/2010
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Announcements Extra Office Hours: Monday 2-3 in BioII 2115; or by appt.
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Review Session Outline Quick Overview of Discussion Topics (12 pts directly from discussion) Blotting Methods, PCR Lac Operon Lambda Phage Poisson Distribution, Mutations, Mutagenic Agents DNA Damage Repair Recombination
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Blotting Methods
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Overview of Southern Blots
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PCR Calculating degeneracy of primers Degenerate primers are used to identify members of a closely related gene- even though AA sequence may be the same, the nucleotide sequence may not
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Example of degeneracy Calculate degeneracy of primers based of aa sequence Lys-Asp-Pro-Ser-Trp DNA sequence: AAA/G-GAT/C-CCT/G/A/C-UCT/G/A/C-TGG Degeneracy: 2 * 2 * 4 * 4 * 1 Degeneracy= 64 Ideally when comparing between primers, want primer with lowest degeneracy number
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analysis What if organism is homozygous for gene? (Agarose/Southern Blot) What if organism is heterozygous for gene? (Agarose/Southern Blot) What if primer dimer occurs? (Agarose DNA Gel) What happens if there is a smear on your gel? (Agarose DNA gel)
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Review_Session2_Part1 - MCDB 101A: Midterm 2 Review Session...

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