LAB 1 - LAB 1 STUDY GUIDE Microscope ability based on ability to 1 Resolve ability of an optical system to form distinguishable images of objects

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LAB 1 – STUDY GUIDE Microscope ability based on ability to: 1. Resolve: ability of an optical system to form distinguishable images of objects separated by small spaces 2. Magnify: apparent enlargement of an object by optical instrument 3. Contrast: use stains to improve opaque regions Magnification of light microscopes range: 100x to 200x Resolving power of best lenses: .2 micrometers (distance between two objects) Resolving power limited by: 1. Wavelength of light being entered: Wavelength = Resolution 450nm(Blue) is lowest wavelength 2. Numerical Aperture (N.A.) : NA = Resolution N.A. of lenses used range from .1(4x) to 1.3(100x) . =(. )( Min Distance Resolved 61 λ )/( . .) N A Microscope: 1. Dissection Microscope: study entire objects in 3D under low res. 2. Compound Light Microscope: Microscope used in lab 3. Electron Microscope: Electrons passed through thin section then reflected on phosphor screen. Specimen is treated with heavy metals
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This note was uploaded on 05/17/2011 for the course MCDB 1AL taught by Professor Bush during the Spring '07 term at UCSB.

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LAB 1 - LAB 1 STUDY GUIDE Microscope ability based on ability to 1 Resolve ability of an optical system to form distinguishable images of objects

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