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LAB 3 - Look at effect of heat and detergent(SDS on...

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LAB 3  – STUDY GUIDE Reaction require  catalysts  – agents that speed up chemical reactions  Reactions occur faster and at lower temperature Biological catalysts called  enzymes  – change reactions kinetics Some RNA molecules, known as  Ribozymes , have catalytic properties SUBSTRATE  (enzyme) PRODUCT Steps to enzymes: 1. Enzymes bind to  active site –  portion that forms non-covalent bonds 2. Enzyme-substrate complex converts substrate to product Colorimetric method:  using a spectro. To measure absorbance LAB: measure activity of  Tyrosinase   enzyme found in mushrooms Tyrosinase  catalyzes formation of melanin – involved in browning of fruits/hair pigments Catalyzes the oxidation of Tyrosine   DOPA   DOPAchrome (colored orange) Metalloprotein:  contains copper as cofactors which mediates electron transfer
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Unformatted text preview: Look at effect of heat and detergent (SDS) on Tyrosinase SDS enzyme is a hydrocarbon with sulfate group attached Amphipathic: both polar and non-polar At low concentrations → binds to proteins making them negatively charged At high concentration → binds to proteins and forms Miscelles Miscelles: internally hydrophobic, externally hydrophilic Bacteriophage: viruses that infect bacteria Recognizes host and inject viral DNA/RNA Host dies and releases more viral particles Restriction Endonucleases: recognize viral genetic material, and degrade them LAB: DNA extracted from bacteriophage lambda Compare the ability of e Endonucleases: HindIII and MspI to digest lambda DNA HindIII: cuts between two A’s MspI: cuts between C’s of CCGG,GGCC...
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