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LAB 4  – STUDY GUIDE Agarose gel electrophoresis:  common way of separating DNA fragments Movement through gel depends on size, shape, charge Thus can be separated Agarose:  complex of branched polysaccharides derived from seaweed – dissolves when  heated Hind III:  made larger, less frequent cuts MspI:  made smaller, more frequent cuts Migration: distance from wells Rf: migration/max. migration Dissecting microscope:  objective lens moves to focus picture rather than stage or specimen Parts of a plant: Haploid egg and 2 haploid sperm fuse creating triploid (3N) – creates  endosperm nutrient source of embryo Within 3 days of fertliziation, pistil elongates and wells to become the  seed pot Pistil: Stigma: where pollen enters Style: where ovaries are kept Ovaries: potential eggs Stamen: Anther: where pollen is stored Filament: rod that projects stamen Life Cycle Of Fruit Fly:
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This note was uploaded on 05/17/2011 for the course MCDB 1AL taught by Professor Bush during the Spring '07 term at UCSB.

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