Chapter 16

Chapter 16 - Chapter16 RestrictionEnzymes...

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Chapter 16 Restriction Enzymes Restriction enzymes:  bacterial defense mechanism towards virus   cut double- stranded DNA molecules Gel electrophoresis:  separates fragments by size 1. Can determine size and sequence DNA Fingerprinting:  works best with highly polymorphic genes 1. Single Nucleotide Polymorphism (SNPs):  inherited variations involving a  single nucleotide base 2. Short Tandem Repeats (STRs):  short, repetitive DNA sequences that occur  side by side on chromosomes Recombinant DNA Sticky Ends:  bond ready ends of restriction fragments o DNA ligase  can permanently join sticky ends   can cut DNA from bacteria and  humans and mix it Clone:  create many copies of a particular gene o Must be  transfected  into host cells   transformed cells are called  transgenic o Reporter gene:  also added in to transfected gene to show some phenotypic 
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This note was uploaded on 05/17/2011 for the course MCDB 101 taught by Professor Unknown during the Spring '08 term at UCSB.

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Chapter 16 - Chapter16 RestrictionEnzymes...

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