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Chem. 262 Notes 9

Chem. 262 Notes 9 - Experiment VIII(Week 2 In this lab you...

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Experiment VIII (Week 2) In this lab you use column chromatography to purify the product of Experiment VIII. Column chromatography affords the partition between a solid support (the solid support is either alumina or silica gel) and solvent as a means of separating and isolating the components of a mixture. The solid adsorbent is contained in a glass tube, and the sample is applied at the top of the column as a solution. The solvent is then allowed to flow through the adsorbent column. The process develops the chromatogram into bands containing the individual compounds. These bands are then eluted in sequence by addition of further solvent to the top of the column. 1. How did you know which fractions contained your desired compound? Out of the four fractions contained from this lab, the first two had identical TLC plates with one dot, so it was assumed that they contained the desired compound. 2. How many spots did you observe on your TLC plate from Week 1? How about from Week 2?
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