Recombinant DNA Technology.ppt

Recombinant DNA Technology.ppt - BIO 325 – Genetics...

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Unformatted text preview: BIO 325 – Genetics Lectures 26 and 27 Recombinant DNA Technology Be of good cheer. Do not think of today’s failures, but of the success that may come tomorrow. You have set yourselves a difficult task, but you will succeed if you persevere; and you will find a joy in overcoming obstacles. Remember, no effort that we make to attain something beautiful is ever lost. ––Helen Keller (Address to the American Association to Promote the Teaching of Speech to the Deaf at Mt. Airy, Philadelphia, Pennsylvania, 8 July 1896). Note: Helen Keller entered Radcliffe College in the fall of 1900 and received her bachelor of arts degree cum laude in 1904. Recombinant DNA technology involves the use of in vitro molecular techniques to isolate and manipulate DNA fragments. Recombinant DNA Technology Recombinant DNA technology involves the use of in vitro molecular techniques to isolate and manipulate DNA fragments. Recombinant DNA molecules are DNA fragments that are covalently linked in the lab. Recombinant DNA Technology Recombinant DNA technology involves the use of in vitro molecular techniques to isolate and manipulate DNA fragments. Recombinant DNA molecules are DNA fragments that are covalently linked in the lab. This technology has enabled researchers to study the relationship between genes and phenotype, and understand gene structure and function. Recombinant DNA Technology Recombinant DNA technology involves the use of in vitro molecular techniques to isolate and manipulate DNA fragments. Recombinant DNA molecules are DNA fragments that are covalently linked in the lab. This technology has enabled researchers to study the relationship between genes and phenotype, and understand gene structure and function. Applications of these technologies include gene therapy and the production of transgenic plants and animals. Recombinant DNA Technology Molecular biologists frequently focus on the structure and function of proteins, or the genes that encode them. Gene Cloning Molecular biologists frequently focus on the structure and function of proteins, or the genes that encode them. Gene cloning involves the isolating and making of many copies of a gene. Gene Cloning Molecular biologists frequently focus on the structure and function of proteins, or the genes that encode them. Gene cloning involves the isolating and making of many copies of a gene. Table 18.1 summarizes some of the uses of gene cloning. Gene Cloning Cloning experiments may involve two kinds of DNA molecules: chromosomal DNA and vector DNA. Gene Cloning Cloning experiments may involve two kinds of DNA molecules: chromosomal DNA and vector DNA. A vector is a small piece of DNA into which a gene of interest is introduced. The vector is then placed within a living cell, where it replicates and produces multiple identical copies of the inserted gene....
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This note was uploaded on 05/19/2011 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas.

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Recombinant DNA Technology.ppt - BIO 325 – Genetics...

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