Genomics II.ppt - BIO 325 Genetics Lectures 33, 34, and 35...

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Unformatted text preview: BIO 325 Genetics Lectures 33, 34, and 35 Genomics II Functional Genomics, Proteomics, and Bioinformatics There are as many ways to live and grow as there are people. Our own ways are the only ways that should matter to us. Evelyn Mandel Functional Genomics, Proteomics, and Bioinformatics Functional genomics strives to understand gene function in a species. Functional Genomics, Proteomics, and Bioinformatics Functional genomics strives to understand gene function in a species. The study of the proteins is called proteomics. The entire collection of proteins in a given species is called the proteome. Functional Genomics, Proteomics, and Bioinformatics Functional genomics strives to understand gene function in a species. The study of the proteins is called proteomics. The entire collection of proteins in a given species is called the proteome. Functional genomics and proteomics can broadly be categorized as being experimental and computational. Functional Genomics, Proteomics, and Bioinformatics Functional genomics strives to understand gene function in a species. The study of the proteins is called proteomics. The entire collection of proteins in a given species is called the proteome. Functional genomics and proteomics can broadly be categorized as being experimental and computational. Bioinformatics attempts to extract information within genetic sequences using a mathematical approach. Functional Genomics, Proteomics, and Bioinformatics Expressed genes can be identified in a cDNA library. Functional Genomics Expressed genes can be identified in a cDNA library. cDNA libraries are made using RNA as the starting material. This is also called an expressed sequence tag (EST) library because the sequences can be used as markers in physical mapping. Functional Genomics cDNA libraries can be used to study gene regulation at the genomic level. Functional Genomics cDNA libraries can be used to study gene regulation at the genomic level. mRNAs are isolated under different conditions. Functional Genomics cDNA libraries can be used to study gene regulation at the genomic level. mRNAs are isolated under different conditions. mRNAs that are found only under a set of conditions indicates genes that are activated under those conditions. Functional Genomics A subtractive cDNA library (also called subtractive hybridization) is shown in this figure. This procedure can be used to isolate genes that are active under a certain set of conditions. Functional Genomics A subtractive cDNA library (also called subtractive hybridization) is shown in this figure. This procedure can be used to isolate genes that are active under a certain set of conditions. Functional Genomics A microarray can identify genes that are transcribed....
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Genomics II.ppt - BIO 325 Genetics Lectures 33, 34, and 35...

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