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Unformatted text preview: BCH 4054 October 29, 1999 HOUR TEST 3 NAME_____________________ Points 1. Many biosynthetic reactions involve the input of energy from ATP and the reducing agent NADPH. For the lipid (fatty acid and sterol) biosynthesis reactions we have studied, identify (by giving reactants and products—structure or name) the steps in which: (12) (a) ATP is utilized in sterol biosynthesis (3 steps). Page Points 1 ____ 2 ____ 3 ____ 4 ____ Total ______ (8) (b) NADPH is utilized in fatty acid biosynthesis (2 steps) (3) 2. Incorporation of [1- 14 C] acetate into fatty acids in crude extracts of liver is stimulated by the addition of bicarbonate ion (i.e., CO 2 in solution at neutral pH), and partial fractionation of the enzymes can demonstrate that bicarbonate is an essential cofactor for the process. But radioactive bicarbonate is not incorporated into the fatty acid chain. Explain these results based on what you know about fatty acid biosynthesis. BCH 4054 --Hour Test 3 Page 2 Name________________________ (6) 3. Describe two roles that citrate has in fatty acid biosynthesis. Describe two roles that citrate has in fatty acid biosynthesis....
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This note was uploaded on 05/22/2011 for the course BCH 4054 taught by Professor Logan/gilmer during the Spring '06 term at FSU.
- Spring '06