Test4(2)

Test4(2) - BCH 4054 HOUR TEST 4 NAME Page(9 1 A circular...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
BCH 4054 April 11, 2001 HOUR TEST 4 NAME_____________________ (9) 1. A circular DNA plasmid of length 1040 bp is supercoiled with a twist (T) value of 100 and a linking number (L) of 94. (a) What is the value of the writhing number (W)? (b) Is the plasmid negatively or positively supercoiled? Page Points 1 ____ 2 ____ 3 ____ 4 ____ 5 ____ Total ______ (c) What effect would topoisomerase I have on L, T, and W? (d) What effect would DNA gyrase and ATP have on L, T, and W? (e) Ethidium bromide is an intercalating agent that inserts between the stacked base pairs, separating the stacks and causing local unwinding that decreases the value of T. What effect would ethidium bromide have on the migration rate of the plasmid during electrophoresis? (f) If part of the plasmid were to undergo a transition from B-DNA to Z-DNA, what would be the effect on L, T, and W? (7) 2. Complete the following table by identifying the DNA structures described: Helix Direction: Left Right Right Base Pairs/Turn 12 10.4 11 Base Tilt 9 o 1.2 o 19 o Diameter (Å) 1.84 2.37 2.55 Name of Structure _______ _______ _______ Which helical structure does double stranded RNA form?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
BCH 4054--Hour Test 4 Page 2 Name________________________ (10) 3. The following proteins have been identified as being involved in homologous recombination in bacteria: RecA, RecB, RecC, RecD, RuvA. RuvB, RuvC. Their synthesis is stimulated when there is extensive DNA damage, and they function to carry out recombination as a
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page1 / 5

Test4(2) - BCH 4054 HOUR TEST 4 NAME Page(9 1 A circular...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online