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FinalExam - BCH 4053 April 23, 2001 FINAL EXAM NAME_ Answer...

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BCH 4053 April 23, 2001 FINAL EXAM NAME_____________________ Answer question 1 or 2 (16 points) 1. Asp-52 and Glu-35 have been shown to be essential residues for catalysis by the enzyme lysozyme. Asp-52 has a pK of 3.5, and Glu-35, being in a hydrophobic environment, has a pK of 6.3. (a) What fraction of each of these residues is protonated at pH 5.0 ? (b) Describe the substrate for lysozyme, and the reaction lysozyme catalyzes (structure or word description). Page Points 1 ____ 2 ____ 3 ____ 4 ____ 5 ____ Total ______ 2. A heptapeptide gave 2,4 dinitrophenylleucine on N-terminal analysis, and free Met as the first product with carboxypeptidase. Trypsin treatment gave a tripeptide (composition: Lys, Leu, Tyr) and a tetrapeptide (composition: Trp, Met, Ala, Glu). Chymotrypsin teatment of the tetrapeptide gave two dipeptides, one migrating to the positive electrode and one migrating to the negative electrode upon electrophoresis at pH 4.5 The dipeptide migrating to the positive electrode showed U.V. absorption at 280 nm (indicating an aromatic residue). What is the sequence of the heptapeptide?
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BCH 4053 –Final Exam Page 2 Name________________________ Answer question 3 or 4 (16 points) 3. Aldolase catalyses the following reaction of glycolysis: fructose-1,6-bisphosphate dihydroxyacetone phosphate + glyceraldehyde-3-phosphate (a) Give the structures of the reactant and products of this reaction. (b)
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This note was uploaded on 05/22/2011 for the course BCH 4053 taught by Professor Logan during the Fall '06 term at FSU.

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FinalExam - BCH 4053 April 23, 2001 FINAL EXAM NAME_ Answer...

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