Pre-Test2

Pre-Test2 - BCH 4053 June 16, 2000 PRE-TEST 2 GROUP NAME _...

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BCH 4053 June 16, 2000 PRE-TEST 2 GROUP NAME ___________________ This test is take-home and open book, and it is intended that all members of the group contribute to completing it. Only one copy is to be submitted by the group, and all members who participated should sign their names below. Test is due at the end of class on Monday, June 26. Please use dark pencil or ink and write legibly . ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ Page Points 1 ______ 2 ______ 3 ______ 4 ______ 5 ______ Total ______ Points (10) 1. Given the following data on four different proteins: Protein M.W.(x10 -3 ) D pI myoglobin 16.9 11.3 7.0 catalase 221.6 4.3 5.6 cytochrome c 13.4 11.4 10.6 serum albumin 68.5 6.1 4.8 Assume all proteins have partial specific volumes of about 0.73 ml/g. Indicate in the blanks the protein(s) with the indicated behavior. ________________________ (a) Difficult to separate on gel-filtration. ________________________ (b) Elutes first from a DEAE column at pH 7.4. (i.e., an anion exchange column). ________________________
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Pre-Test2 - BCH 4053 June 16, 2000 PRE-TEST 2 GROUP NAME _...

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