Pre-Test4

Pre-Test4 - BCH 4053 July 21, 2000 PRE-TEST 4 GROUP NAME _...

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BCH 4053 July 21, 2000 PRE-TEST 4 GROUP NAME ___________________ This test is take-home and open book, and it is intended that all members of the group contribute to completing it. Only one copy is to be submitted by the group, and all members who participated should sign their names below. Test is due at the end of class on Monday, July 31. Please use dark pencil or ink and write legibly . ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ Page Points 1 ______ 2 ______ 3 ______ 4 ______ 5 ______ Total ______ Points (8) 1. The pH-rate profile for ribonuclease is bell shaped, with a pH maximum at 6.0 and inflection points of approximately 5.8 and 6.2 in the two sides of the curve. (a) What amino acid side chain(s) would be likely candidates for titration at these pK values? (b) Presumably the groups responsible for these pH effects would be involved in general-acid or general-base catalysis in the mechanism. Identify by pK (5.8 or 6.2) the
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Pre-Test4 - BCH 4053 July 21, 2000 PRE-TEST 4 GROUP NAME _...

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