Test1(2)

Test1(2) - BCH 4053 June 2, 2000 (10) 1. HOUR TEST 1 NAME_...

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BCH 4053 June 2, 2000 HOUR TEST 1 NAME_____________________ (10) 1. Carbonic Acid in solution is an equilibrium mixture of H 2 CO 3 and dissolved CO 2 . If its standard state is taken a 1 M for the sum of both forms, then pK 1 for the following reaction is 6.1: H 2 CO 3 (total) H + + HCO 3 - (a) Calculate the [HCO 3 - ]/[H 2 CO 3 (total) ] ratio in blood at pH 7.4. Page Points 1 ____ 2 ____ 3 ____ 4 ____ Total ______ (b) Normally buffers have their best buffering capacity at their pK. Yet bicarbonate buffer is a very good buffer at pH 7.4, even better than at its pK of 6.1. Explain why. 2. Draw the structure of the following peptide, showing all dissociable functional groups in their fully protonated form, and indicate the approximate pK of each of the dissociable functional groups: (10) (a) Lys.Cys.Arg.Tyr.Glu (1) (b) What is the charge on the fully protonated form of the peptide? (1) (c) What is the charge on the fully unprotonated form of the peptide? (1) (d) What is the approximate pI of the peptide? (1) (e) What is the net charge on the peptide at pH 6.0?
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BCH 4053 --Hour Test 1 Page 2 Name________________________ (12) 3. Formic acid has a pK of 3.75. How many mL of 0.1 M NaOH must be added to 100 mL of 0.05 M formic acid to make a buffer solution with a pH of 4.25? (20) 4. Identify the following amino acids by giving the name , the three letter abbreviation , and the one letter abbreviation . The number of amino acids in each category is given in parenthesis. Category
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Test1(2) - BCH 4053 June 2, 2000 (10) 1. HOUR TEST 1 NAME_...

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